Preparation of active animal amylase and process of making same



Patented Feb, 6,

RICHARD KERN AND GEORGES JENNY,

SWISS FERMEN '1 COMPANY,

F BASEL, SWITZERLAND, ASSIGNORS TO LTD., OF BASEL, SWITZERLAND.

- PREPARATION OF ACTIVE ANIMAL AMYLASE AND PROCESS 01E MAKING SAIEE.

No Drawing.

To all whom it may concern:

Application filed November Be it known that we, RIOHARD KERN and GEORGES JENNY, both citizens of the Swiss Republic, and resi dents of Basel, Switzer- 5 land,'have invented a new and durable Preparation of Active Animal Amylase and Process of Making Same, of which the following is a full, clear,

and exact specification.

Attempts have lately been made to apply for pharmaceutica the amylases obtain The attempts have 1 and technical purposes able from animal organs.

been more or less frustrated hitherto by the want of permanence of such preparations,

and of the dilute aqueous solutions made from them, so that practical application of them been practicable.

By the present inventi limited durabilit on a large scale has not on a practically unis imparted to the animal amylases by ad mg in suitable manner in the course of their separation and preparation organic colloids soluble in water, such as gum, gelatine, gummy and gelatinous sub stances and their degradation products,

mucous substances, soluble albumin deriva tives, soluble starches and dextrins. These colloidal substances act as protective colloids on the amylase, since they, to a certain degree, encase this and exert a protective influence whereby the gradual inactivation by chemical and physical effects of the ambient room hitherto unavoidable are prevented.

In many cases they simultaneously automatically adjust the ion concentration most sultable for the preservation and application of the am obviously be aided able chemicals in k ylas'e, which adjustment can by the addition of suitnown manner. This protective effect is exhibited both in solution and in solid that, for instance,

preparations and is so deeply seated a preparation made in the known manner by drying an amylase solution on an inorganic salt, or on an indlfferent absorbing material, retained fully and completely its original amylolytic capacity in presence of a protective colloid during. an observation period of 14 months,

whereas a similar actly the same manner,

preparation made in exbut without the pro- "tectmg colloid,-lostalready after 2 months at least g rd and after 8 of its amylolytic become useless. I solution 1n presen months per cent.

m'ilarly, a dilute aqueous ce of'o'ne of the above ower, and had thereforev 23, 1921. Serial 1\T0. 517,309.

named protective colloids, retained its full activity for an observation period of 3 months and more, whereas the same solution without a colloid, even under favourable temperature conditions, had lost irrecoverablywithin a few hours the greater part of its activity. By choice of the kind and proportion of the organic' protective colloid in question, the most important consideration on practical grounds is the solubllity of the colloid in water, so that its protective action may be exerted not only during the manufacture and preservation of the preparation but also during its technical or therapeutical use, that is to say its use in aqueous solution. For certain purposes dry preparations of the animal organs containing amylase, or extracts from these organs, dried on convenient substances, or precipitates containing amylase and extracts of the said organs evaporated at low temperature,- in each case with addition of the aforesaid protective colloids, are best suited; for other purposes liquid preparations containing the protective colloids in solution and preserved in a suitable manner are more advantageous.

The following example illustrates the invention:

An active aqueous extract from an animal organ containing amylase (salivary glands, pancreas or the like) is evaporated in a vacuum at the lowest possible temperature to the desired degree of amylase concentration and is brought into the dry form in known mannor on an inorganic salt, such as sodium phosphateor sodium sulfate with or without addition of a protective colloid, such as gum arabic; in this operation high temperature is avoided as much as possible.

For enhancing the durability and also to secure a suflicient percentage content of protective colloid for later use in aqueous solution, the preparation prepared as described above, so far as it does not contain suflicient proportion of protective colloid, may be stirred at a moderate temperature, for instance 40 C'. with a concentrated solution of a protective colloid, such as gum arabic, until a homogeneous mixture is obtained; it may then be allowed to solidify and dried in a vacuum. The temperature named and\ 105 the proportions, as well as the kind of materials used and their preparation, are given only by way of example and may be modified according to the intended application of the amylase preparation'and to its final form.

What We claim is:

1. The herein described durable preparation of animal amylase, consisting of active animal amylase and an organic protective colloid soluble in water.

2. The herein described durable preparation of animal amylase, consisting of an aqueous extract containing active animal amylase andan organic protective colloid soluble in water in solution in the said extract.

3. The herein described durable preparation of animal amylase, consisting of a dry extract of active animal amylase, each of its particles being coated with an. organic protective colloid soluble in water.

4. The herein described process for the manufacture of a durable preparation of animal amylase, consisting in preparing an aqueous extract of active animal amylase from an animal organ and adding to the said extract an organic protective colloid soluble in Water.

. 5. The herein described process for the manufacture of a durable preparation of animal amylase, consisting in preparlng an animal organ, adding to the said extract an aqueous extract of animal amylase from an organic protective colloid soluble in water and drying the resulting solution.

6. The herein described process for the manufacture of a durable preparation of animal amylase, consisting in preparing an aqueous extract of animal amylase from an animal organ, evaporating the said extract to dryness, adding to the resulting dry extract a concentrated aqueous solution of an organic protective colloid, stirring the resulting mixture and letting it stand until it solidifies. Y

7. The herein described process for the manufacture of a durable preparation of animal amylase, consisting in preparing an aqueous extract of an animal amylase from an animal organ, evaporating the said extract to dryness, adding to the resulting dry extract a concentrated aqueous solution of an organic protective colloid, stirring the resulting mixture and evaporating it to dryness.

In witness whereof We have hereunto signed our names this 4th day of November- 1921, in the presence of two subscribing wit- 

